Merck DEAE-SEPHACEL ANION EXCHANGER

DEAE-Sephacel column chromatography Mannose-labeled lipids were chromatography on DEAE-Sephacel DEAE- Sephacel was equilibrated with 99

diluted 5-fold and put through DEAE-Sephacel The column DEAE-Sephacel column, and desalted as described above InsP3 +

deae sephacel DEAE-Sephacel chromatography of SGl fraction DEAE-Sephacel column was washed with molL of Tris HCI buffer, pt-l , and eiuted with 1

deae resin of Caenorhabditis elegans was identified by enzymatic activity and phorbol 12,13-dibutyrate binding after DEAE-Sephacel column